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J. Trop. Resour. Sustain. Sci. 3 (2015) 129-131

Acclimatization of Eurycoma longifolia (Tongkat Ali) Plantlets to Ex Vitro Conditions

Muhammad Fuad Yahya1*, Nor Hasnida Hassan1, Nazirah Abdullah1, Siti Suhaila Abd. Rahman1, Haliza Ismail1, Mohd Zaki Abdullah1, Farah Fazwa Mohd Ariff1, Mohamad Lokmal Ngah1, Rosdi Koter1, Rozidah Khalid1, Rohani Abdullah1, Norfarhana Zakaria2, Norhidayah Zakaria2

1Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia
2Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia

*Email Address : This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract : Eurycoma longifolia is one of the famous herbal plants with great medicinal benefits. The plant which also known as Tongkat Ali is well-known for treating erectile dysfunction and as energy booster. Tongkat Ali plantlets were produced using in vitro propagation technique to cater the demand for planting material. The development of successful acclimatization technique is prerequisite for in vitro propagation method. Acclimatization is a step to prepare the plantlets to survive and grow in different environment compare to laboratory. In this study, an attempt was made to identify the best potting media to acclimatize Tongkat Ali using glass chamber. Rooted plantlets about 2-4 cm height were used and subsequently transferred to different potting media for acclimatization i.e jiffy 7, sand, baked soil, mixture of cocoa peat and sand, sand and top soil and lastly cocoa peat and baked soil. Plantlets grown in jiffy 7 showed 100% survival followed by the mixture of cocoa peat and baked soil with 94.45% of survival. The acclimatized plantlets were transplanted into polybag and maintained in the shadehouse condition.

Keywords : Eurycoma longifolia,Tongkat Ali,acclimatization